近日由国家海洋局第一海洋研究所郑明刚老师撰写的有关研究不同生长时期微拟球藻转录组测序的论文已在Elsevier科学出版公司《Gene》期刊正式发表。派森诺生物在此送上诚挚的祝贺！

该研究项目对象微拟球藻是公认的高产油海水藻，它的油脂含量占干重的比重达68%以上，是进行生物柴油炼制的理想之选。研究表明处于稳定生长期的微拟球藻其细胞中的脂质含量显著高于处于对数生长期的。

利用Illumina HiSeq2000平台对处于稳定生长期和对数生长期的微拟球藻进行转录组测序，得到其全转录组信息，寻找参与脂类代谢的基因，通过表达差异分析，发现7305个unigene在对数生长期表达上调，9745个unigene在稳定生长期表达上调，其中有20个与脂类代谢相关。这些基因的发现有助于进一步研究微藻的油脂积累，为利用基因改造的方法提高微藻油脂积累能力奠定基础。

原文摘要：
Nannochloropsis sp. is an oil-rich microalga. The lipid content of cells at stationary growth phase was much higher than that of cells at logarithm growth phase, but very little information focusing on the abundances of gene transcripts concerning lipid accumulation is available. In present study, the second generation Illumina sequencing was used to investigate the genes and pathways of Nannochloropsis sp. in order to identify those involved in lipid metabolism. In total, 29,203 unigenes were obtained from logarithm and stationary growth phases. The transcripts of some unigenes were differentially abundant at two growth phases. Among the unigenes obtained, about 195 unigenes were involved in lipid metabolism and 315 unigenes were putatively transcription factors. These genes are a very useful resource for the future studies of Nannochloropsis sp.

原文索引：[Minggang Zheng,e al.Transcriptome sequencing, annotation and expression analysis of Nannochloropsis sp. at different growth phases.Gene,2013,523(2):117-121]