慢病毒包装质粒 pCMV-dR8.91
产品名称： 慢病毒包装质粒 pCMV-dR8.91
英文名称： pCMV-dR8.91
产品编号： JY03033
产品价格： 680
产品产地： 江苏南京
品牌商标： 江苑生物
更新时间： 2025-10-24T10:19:18
使用范围： null
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- 地址 : 江北新区智能制造产业园（智合园）科创大道9号A2栋
- 邮编 : 210000
- 所在区域 : 江苏
- 电话 : 177****6632 点击查看
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- 邮箱 : jiangyuanbio@163.com
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Locked="false" SemiHidden="true" UnhideWhenUsed="true" Name="Smart Link"/> </w:LatentStyles> </xml><![endif]--><!-- [if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:普通表格; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-pagination:widow-orphan; font-size:10.5pt; mso-bidi-font-size:11.0pt; font-family:等线; mso-ascii-font-family:等线; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:等线; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:等线; mso-hansi-theme-font:minor-latin; mso-font-kerning:1.0pt;} </style> <![endif]--> <p><strong><span style="color: #2e74b5;">质粒信息</span></strong></p> <table width="473"> <tbody> <tr> <td width="113"> <p>质粒名称：</p> </td> <td width="359"> <p><strong>pCMV-dR8.91</strong></p> </td> </tr> <tr> <td width="113"> <p>质粒全长：</p> </td> <td width="359"> <p>12150 bp</p> </td> </tr> <tr> <td width="113"> <p>载体骨架：</p> </td> <td width="359"> <p> </p> </td> </tr> <tr> <td width="113"> <p>载体类型：</p> </td> <td width="359"> <p>哺乳动物表达，慢病毒；包装质粒</p> </td> </tr> <tr> <td width="113"> <p>插入序列：</p> </td> <td width="359"> <p>gag/pol/rev/tat</p> </td> </tr> <tr> <td width="113"> <p>筛选标记：</p> </td> <td width="359"> <p>None</p> </td> </tr> <tr> <td width="113"> <p>荧光标记：</p> </td> <td width="359"> <p>None</p> </td> </tr> <tr> <td width="113"> <p>克隆方法：</p> </td> <td width="359"> <p> </p> </td> </tr> <tr> <td width="113"> <p>启动子：</p> </td> <td width="359"> <p>CMV</p> </td> </tr> <tr> <td width="113"> <p>5’测序引物：</p> </td> <td width="359"> <p>CMV-F：CGCAAATGGGCGGTAGGCGTG</p> </td> </tr> <tr> <td width="113"> <p>3’测序引物：</p> </td> <td width="359"> <p>SP6：ATTTAGGTGACACTATAGAA</p> </td> </tr> <tr> <td width="113"> <p>诱导方式：</p> </td> <td width="359"> <p>无需诱导</p> </td> </tr> <tr> <td width="113"> <p>细菌抗性：</p> </td> <td width="359"> <p>Ampicillin</p> </td> </tr> <tr> <td width="113"> <p>感受态：</p> </td> <td width="359"> <p>Stbl3</p> </td> </tr> <tr> <td width="113"> <p>生长温度：</p> </td> <td width="359"> <p>37°C</p> </td> </tr> <tr> <td width="113"> <p>拷贝数：</p> </td> <td width="359"> <p>高拷贝</p> </td> </tr> <tr> <td width="113"> <p>质粒用途：</p> </td> <td width="359"> <p>pCMV-dR8.9，也被称为delta 8.9或者Δ8.9，是慢病毒包装的辅助质粒，载有病毒gag，pol，rev和tat基因。pCMV-dR8.9与pCMV-VSV-G构成第二代慢病毒包装系统，用于包装第二代或者第三代慢病毒载体质粒。pCMV-dR8.9、pCMV-VSV-G和慢病毒载体质粒这3个质粒组成三质粒系统（即第二代慢病毒系统）。将这3个质粒共同转染293T等细胞以包装重组慢病毒，用此慢病毒感染目的细胞，即可实现基因敲除、敲降和过表达等。</p> </td> </tr> </tbody> </table> <p><strong><span style="color: #2e74b5;">相关产品</span></strong></p> <table width="643"> <tbody> <tr> <td width="161"> <p><span style="color: black;">产品编号</span></p> </td> <td width="321"> <p><span style="color: black;">产品名称</span></p> </td> <td width="161"> <p><span style="color: black;">产品规格</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03011/JY03012</span></p> </td> <td width="321"> <p>慢病毒浓缩试剂盒</p> </td> <td width="161"> <p><span style="color: black;">50 mL/100 mL</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03013/JY03014</span></p> </td> <td width="321"> <p>PEG-8000慢病毒浓缩液</p> </td> <td width="161"> <p><span style="color: black;">50 mL/100 mL</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03061/JY03062</span></p> </td> <td width="321"> <p>全套第二代慢病毒包装质粒（三质粒系统）</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03063/JY03064</span></p> </td> <td width="321"> <p>全套第三代慢病毒包装质粒（四质粒系统）</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03027/JY03028</span></p> </td> <td width="321"> <p>慢病毒包装质粒 pMD2.G</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03029/JY03030</span></p> </td> <td width="321"> <p>慢病毒包装质粒 psPAX2</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03031/JY03032</span></p> </td> <td width="321"> <p>慢病毒包装质粒 pCMV-VSV-G</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03033/JY03034</span></p> </td> <td width="321"> <p>慢病毒包装质粒 pCMV-dR8.91</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03035/JY03036</span></p> </td> <td width="321"> <p>慢病毒包装质粒 pMDLg/pRRE</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03037/JY03038</span></p> </td> <td width="321"> <p>慢病毒包装质粒 pRSV-Rev</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10011</span></p> </td> <td width="321"> <p>pLKO.1 puro</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10012</span></p> </td> <td width="321"> <p>pLKO.1-EGFP-Puro</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10013</span></p> </td> <td width="321"> <p>pLVX-Puro</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY10014</span></p> </td> <td width="321"> <p>pLenti-puro</p> </td> <td width="161"> <p><span style="color: black;">1 μg/100 μg</span></p> </td> </tr> <tr> <td width="161"> <p><span style="color: black;">JY03041</span></p> </td> <td width="321"> <p>293T，HEK-293T，人胚肾细胞</p> </td> <td width="161"> <p><span style="color: black;">> 1×10⁶ cells</span></p> </td> </tr> </tbody> </table>