人转录误调节在恶性血液肿瘤中的定量PCR芯片试剂盒描述：

ScienCells GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Kit (GQH-TMH) is designed to facilitate gene expression profiling of 88 key genes involved in three types hematological malignancy development (acute myeloid leukemia, precursor B lymphoblastic leukemia, and precursor T lymphoblastic leukemia), and is based on extensive literature research. Brief examples of how included genes may be grouped according to function and malignancy types are shown below:     

• Cell cycle regulation: CCNT1, CCNT2, CDK9, CDK14, CDKN2C
• Signaling mediation: IGF1R, BMP2K, PROM1
• Differentiation regulation: CSF1R, CD14, DUSP6, GZMB, HOXA9, CCR7, FUT8, SIX4, EYA1
• Mutated transcription factors identified
  • in acute myeloid leukemia: AML1, CEBPA, PLZF, ZBTB16, SPI1
  • in precursor B lymphoblastic leukemia: TCF3, PBX1
  • in precursor T lymphoblastic leukemia: TLX1, TLX3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Plate Layout*

(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development

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